Skip to Main content Skip to Navigation
Journal articles

Genetic variation and drought response in two Populus X euramericana genotypes through 2-DE proteomic analysis of leaves from field and glasshouse cultivated plants

Abstract : Genotype and water deficit effects on leaf 2-DE protein profiles of two Populus deltoides x Populus nigra, cv. 'Agathe_F' and 'Cima', were analysed over a short-term period of 18 days in glasshouse using 4-month-old rooted cuttings and over a long-lasting period of 86 days in open field using 4-year-old rooted cuttings. Leaf proteomes were analyzed using two-dimensional gel electrophoresis, and proteins were identified after database searching from MS peptide spectra. A reliable genotype effect was observed in the leaf proteome over experiment locations, water regimes and sampling dates. Quantitative differences between genotypes were found. Most of them corresponded to proteins matching isoforms or post-translational modification variants. However, 'Cima' displayed the highest abundance of antioxidant enzymes. In response to water deficit. about 10% of the reproducible spots significantly varied regardless of the experiment location, among which about 25% also displayed genotype-dependent variations. As a whole, while 'Cima' differed from 'Agathe_F' by increased abundance of enzymes involved in photorespiration and in oxidative stress, 'Agathe_F' was mainly differentiated by increased abundance of enzymes involved in photosynthesis.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02656637
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Friday, May 29, 2020 - 11:46:14 PM
Last modification on : Tuesday, October 12, 2021 - 5:20:32 PM

Identifiers

Citation

Ludovic Bonhomme, R. Monclus, Delphine Vincent, Sabine Carpin, S. Claverol, et al.. Genetic variation and drought response in two Populus X euramericana genotypes through 2-DE proteomic analysis of leaves from field and glasshouse cultivated plants. Phytochemistry, Elsevier, 2009, 70 (8), pp.988-1002. ⟨10.1016/j.phytochem.2009.05.013⟩. ⟨hal-02656637⟩

Share

Metrics

Record views

72