Genetic variation and drought response in two Populus X euramericana genotypes through 2-DE proteomic analysis of leaves from field and glasshouse cultivated plants - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Phytochemistry Année : 2009

Genetic variation and drought response in two Populus X euramericana genotypes through 2-DE proteomic analysis of leaves from field and glasshouse cultivated plants

Résumé

Genotype and water deficit effects on leaf 2-DE protein profiles of two Populus deltoides x Populus nigra, cv. 'Agathe_F' and 'Cima', were analysed over a short-term period of 18 days in glasshouse using 4-month-old rooted cuttings and over a long-lasting period of 86 days in open field using 4-year-old rooted cuttings. Leaf proteomes were analyzed using two-dimensional gel electrophoresis, and proteins were identified after database searching from MS peptide spectra. A reliable genotype effect was observed in the leaf proteome over experiment locations, water regimes and sampling dates. Quantitative differences between genotypes were found. Most of them corresponded to proteins matching isoforms or post-translational modification variants. However, 'Cima' displayed the highest abundance of antioxidant enzymes. In response to water deficit. about 10% of the reproducible spots significantly varied regardless of the experiment location, among which about 25% also displayed genotype-dependent variations. As a whole, while 'Cima' differed from 'Agathe_F' by increased abundance of enzymes involved in photorespiration and in oxidative stress, 'Agathe_F' was mainly differentiated by increased abundance of enzymes involved in photosynthesis.

Dates et versions

hal-02656637 , version 1 (29-05-2020)

Identifiants

Citer

Ludovic Bonhomme, R. Monclus, Delphine D. Vincent, Sabine Carpin, S. Claverol, et al.. Genetic variation and drought response in two Populus X euramericana genotypes through 2-DE proteomic analysis of leaves from field and glasshouse cultivated plants. Phytochemistry, 2009, 70 (8), pp.988-1002. ⟨10.1016/j.phytochem.2009.05.013⟩. ⟨hal-02656637⟩
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