Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Gradual soil water depletion results in reversible changes of gene expression, protein profiles, ecophysiology and growth performance in populus euphratica, a poplar growing in arid regions

Abstract : The responses of Populus euphratica Oliv. plants to soil water deficit were assessed by analyzing gene expression, protein profiles, and several plant performance criteria to understand the acclimation of plants to soil water deficit. Young, vegetatively propagated plants originating from an arid, saline field site were submitted to a gradually increasing water deficit for 4 weeks in a greenhouse and were allowed to recover for 10 d after full reirrigation. Time-dependent changes and intensity of the perturbations induced in shoot and root growth, xylem anatomy, gas exchange, and water status were recorded. The expression profiles of approximately 6,340 genes and of proteins and metabolites (pigments, soluble carbohydrates, and oxidative compounds) were also recorded in mature leaves and in roots (gene expression only) at four stress levels and after recovery. Drought successively induced shoot growth cessation, stomatal closure, moderate increases in oxidative stressrelated compounds, loss of CO2 assimilation, and root growth reduction. These effects were almost fully reversible, indicating that acclimation was dominant over injury. The physiological responses were paralleled by fully reversible transcriptional changes, including only 1.5% of the genes on the array. Protein profiles displayed greater changes than transcript levels. Among the identified proteins for which expressed sequence tags were present on the array, no correlation was found between transcript and protein abundance. Acclimation to water deficit involves the regulation of different networks of genes in roots and shoots. Such diverse requirements for protecting and maintaining the function of different plant organs may render plant engineering or breeding toward improved drought tolerance more complex than previously anticipated
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [84 références]  Voir  Masquer  Télécharger

https://hal.inrae.fr/hal-02658086
Déposant : Migration Prodinra <>
Soumis le : samedi 30 mai 2020 - 11:38:34
Dernière modification le : mercredi 18 novembre 2020 - 09:00:08

Fichier

Bogeat2007PlantPhysiol_1.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

  • HAL Id : hal-02658086, version 1
  • PRODINRA : 12744

Collections

Citation

Marie-Béatrice Bogeat-Triboulot, Mikael Brosché, Jenny Renaut, Laurent Jouve, Didier Le Thiec, et al.. Gradual soil water depletion results in reversible changes of gene expression, protein profiles, ecophysiology and growth performance in populus euphratica, a poplar growing in arid regions. Plant Physiology, American Society of Plant Biologists, 2007, 143 (2), pp.876-892. ⟨hal-02658086⟩

Partager

Métriques

Consultations de la notice

14

Téléchargements de fichiers

39