HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Expression profiling of Botrytis cinerea genes identifies three patterns of up-regulation in planta and an FKBP12 protein affecting pathogenicity

Abstract : The ascomycete Botrytis cinerea is a broad-spectrum plant pathogen. Here, we describe the first macroarray transcriptomic study of the fungus in realtime infection conditions. Infection of Arabidopsis thaliana leaves by B. cinerea was monitored using macroarrays, containing 3032 genes. Variance analysis revealed that 7% of B. cinerea genes are differentially expressed during infection and allowed us to identify 27 genes significantly up-regulated in planta. Among them, two genes have already been associated with fungal pathogenicity, while eight genes have unidentified functions. The 27 genes were separated into three groups according to their expression profile. The first group showed maximal expression at the early stage following fungal penetration, the second one showed maximal expression at the outset of the colonization of plant leaves and the third group showed maximal expression when the colonization of plant leaves was completed. A gene of the last group (BcPIC5), which is homologous to FKBP12 proteins, was disrupted in order to determine its role in pathogenicity. At seven days post-inoculation, the lesions caused by the Delta BcPIC5 mutant on bean leaves were reduced by 69% and did not further expand compared to the wild-type. These results confirm that transcriptomic analysis under infection conditions can be very valuable for the identification of fungal genes related to pathogenicity. (c) 2006 Elsevier Ltd. All rights reserved.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02658200
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Saturday, May 30, 2020 - 11:51:52 AM
Last modification on : Thursday, April 29, 2021 - 11:36:04 AM

Links full text

Identifiers

Collections

Citation

Anastasia Gioti, Adeline Simon, Pascal Le Pecheur, Corinne Giraud-Delville, Jean Marc Pradier, et al.. Expression profiling of Botrytis cinerea genes identifies three patterns of up-regulation in planta and an FKBP12 protein affecting pathogenicity. Journal of Molecular Biology, Elsevier, 2006, 358 (2), pp.372-386. ⟨10.1016/j.jmb.2006.01.076⟩. ⟨hal-02658200⟩

Share

Metrics

Record views

9