High quality draft sequences for prokaryotic genomes using a mix of new sequencing technologies - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles BMC Genomics Year : 2008

High quality draft sequences for prokaryotic genomes using a mix of new sequencing technologies

Abstract

Massively parallel DNA sequencing instruments are enabling the decoding of whole genomes at significantly lower cost and higher throughput than classical Sanger technology. Each of these technologies have been estimated to yield assemblies with more problematic features than the standard method. These problems are of a different nature depending on the techniques used. So, an appropriate mix of technologies may help resolve most difficulties, and eventually provide assemblies of high quality without requiring any Sanger-based input. We compared assemblies obtained using Sanger data with those from different inputs from New Sequencing Technologies. The assemblies were systematically compared with a reference finished sequence. We found that the 454 GSFLX can efficiently produce high continuity when used at high coverage. The potential to enhance continuity by scaffolding was tested using 454 sequences from circularized genomic fragments. Finally, we explore the use of Solexa-Illumina short reads to polish the genome draft by implementing a technique to correct 454 consensus errors. High quality drafts can be produced for small genomes without any Sanger data input. We found that 454 GSFLX and Solexa/Illumina show great complementarity in producing large contigs and supercontigs with a low error rate.

Domains

Biotechnology
Fichier principal
Vignette du fichier
2008_Aury_BMC_Genomics_1.pdf (1011.25 Ko) Télécharger le fichier
Origin Publisher files allowed on an open archive
Loading...

Dates and versions

hal-02659381 , version 1 (30-05-2020)

Identifiers

Cite

Jean-Marc Aury, Corinne Cruaud, Valérie Barbe, Odile Rogier, Sophie Mangenot, et al.. High quality draft sequences for prokaryotic genomes using a mix of new sequencing technologies. BMC Genomics, 2008, 9, ⟨10.1186/1471-2164-9-603⟩. ⟨hal-02659381⟩
23 View
32 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More