Ten commonly cultivated cultivars of tomato (Solanum lycopersicum L.) in Nepal were tested in a plastic tunnel and in the field for their susceptibility to Pseudomonas syringae pv. tomato, the causal agent of bacterial speck. Four of the ten cultivars were local and six were hybrids. The leaves of each cultivar were sprayed with P. s. pv. tomato both in the tunnel and in the field. The genotypes exhibited a considerable variation in response to infection, with the disease severity index (DSI) varying from 1.80 to 4.25 in the field and from 1.10 to 4.20 in the tunnel. The cultivars Thims 16, C.L. and Spectra 737 were the least susceptible in the field, with DSI values of 1.80, 2.05 and 2.25, respectively; while in the tunnel all the local cultivars (C.L., Panjabi, B.L. and Lapsi Gede) showed very low susceptibility, with respective DSI values of 1.10, 1.20, 1.65 and 2.30. In the field, the most susceptible cultivar was Lapsi Gede (DSI=4.25) and in the tunnel the most susceptible was NS719 (DSI= 4.20).