Skip to Main content Skip to Navigation
Journal articles

Cryopreservation of Pelargonium apices by droplet-vitrification

Abstract : The droplet-vitrification method was adapted to Pelargonium apices by optimizing the duration of the loading solution (LS) as well as the plant vitrification solution 2 (PVS2). The excised apices were dehydrated in two steps (20 min in LS and 15 min in PVS2) and then immersed directly in liquid nitrogen (LN). After thawing and unloading in the recovery solution at room temperature for 15 min, apices were plated onto semi-solid Murashige and Skoog medium. This simple protocol without any pretreatment was successfully applied to eight cultivars with a survival level ranging between 55.6 - 96.2 % and a regrowth level between 9.1% - 70.6%. These results prove the feasibility of the long-term storage of Pelargonium germplasm through cryopreservation
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02664931
Contributor : Migration ProdInra Connect in order to contact the contributor
Submitted on : Sunday, May 31, 2020 - 5:45:36 AM
Last modification on : Thursday, May 5, 2022 - 4:54:03 PM

Identifiers

  • HAL Id : hal-02664931, version 1
  • PRODINRA : 20526
  • WOS : 000257824900007

Citation

Anthony Gallard, Bart Panis, Noëlle Dorion, Rony Swennen, Agnès Grapin. Cryopreservation of Pelargonium apices by droplet-vitrification. Cryo Letters, Cryo Letters, 2008, 29 (3), pp.243-251. ⟨hal-02664931⟩

Share

Metrics

Record views

5