Rational design of <em>Bacillus stearothermophilus</em> US100 L-arabinose isomerase: potential applications for D-tagatose production - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Biochimie Année : 2009

Rational design of Bacillus stearothermophilus US100 L-arabinose isomerase: potential applications for D-tagatose production

Résumé

L-arabinose isomerases catalyze the bioconversion of D-galactose into D-tagatose. With the aim of producing an enzyme optimized for D-tagatose production, three Bacillus stearothermophilus US100 L-arabinose isomerase mutants were constructed, purified and characterized. Our results indicate that mutant Q268K was significantly more acidotolerant and more stable at acidic pH than the wild-type enzyme. The N175H mutant has a broad optimal temperature range from 50 to 65 degrees C. With the aim of constructing an acidotolerant mutant working at relatively low temperatures we generated the Q268K/N175H construct. This double mutant displays an optimal pH in the range 6.0-7.0 and an optimal activity around 50-65 degrees C, temperatures at which the enzyme was stable without addition of metal ions.

Dates et versions

hal-02665601 , version 1 (31-05-2020)

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Citer

Moez M. Rhimi, Nushin Aghajari, Michel Juy, Hichem Chouayekh, Emmanuelle Maguin, et al.. Rational design of Bacillus stearothermophilus US100 L-arabinose isomerase: potential applications for D-tagatose production. Biochimie, 2009, 91 (5), pp.650-653. ⟨10.1016/j.biochi.2009.01.014⟩. ⟨hal-02665601⟩
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