HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Identification of the zebrafish IFN receptor: Implications for the origin of the vertebrate IFN system

Abstract : The recent description of virus-induced fish IFNs has raised questions about the evolution of this complex antiviral system. Identification of the receptor of the zebrafish virus-induced IFN (zIFN) was sought to help resolve these questions. We set up an experimental system to study the zIFN system in the course of a viral infection of zebrafish embryos. In this setting, zIFN was induced by viral infection, and we identified zIFN-dependent induced transcripts. Embryos quickly died from the infection, but zIFN overexpression increased their survival. We took advantage of this experimental system to perform in vivo loss and gain of function analysis of candidate receptors of the class II helical receptor family and identified zCRFB1 and zCRFB5 as the two subunits of the zebrafish IFN receptor. Based on the organization of the zIFN gene and the protein structure of the identified receptor components, the virus-induced fish IFNs appear as orthologs of mammalian IFN-_, specifying type III IFN as the ancestral antiviral system of vertebrates.
Keywords : ZEBRAFISH
Document type :
Journal articles
Complete list of metadata

Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Sunday, May 31, 2020 - 9:17:10 AM
Last modification on : Thursday, April 7, 2022 - 10:10:40 AM


  • HAL Id : hal-02666713, version 1
  • PRODINRA : 144735
  • WOS : ISI:000245197300045


Jean-Pierre Levraud, Pierre Boudinot, Ingrid Colin, Abdenour Benmansour, Nadine Peyriéras, et al.. Identification of the zebrafish IFN receptor: Implications for the origin of the vertebrate IFN system. Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2007, 178 (7), pp.4385-4394. ⟨hal-02666713⟩



Record views