Adaptation to pyrrolnitrin in<em> Botrytis cinerea</em> and cost of resistance - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Plant Pathology Année : 2010

Adaptation to pyrrolnitrin in Botrytis cinerea and cost of resistance

Sakhr Ajouz
  • Fonction : Auteur
  • PersonId : 776124
  • IdRef : 139878025
Philippe C. Nicot
Marc Bardin

Résumé

The objective of this work was to estimate the risk of a decrease in the efficacy of biocontrol as a result of selection pressure exerted by biocontrol agents on Botrytis cinerea, focusing on pyrrolnitrin, an antibiotic identified in diverse biocontrol agents having an effect on B. cinerea. To evaluate a possible decrease in sensitivity to pyrrolnitrin, 10 successive generations of five isolates of B. cinerea were produced in vitro in the presence of a sublethal dose (10 mu g L-1) of the antibiotic. For one isolate, a significant reduction in the sensitivity to pyrrolnitrin at the fifth generation was observed with a resistance factor of c. 11. The production of 10 additional generations for four of these isolates, with increasing doses of pyrrolnitrin (100-4000 mu g L-1), resulted in the development of variants of B. cinerea with high levels of resistance to the antibiotic (RF > 1000) and a reduced sensitivity in vitro to a pyrrolnitrin-producing bacterium. Reverse adaptation of resistant variants after 10 additional generations in the absence of selection pressure was not observed, suggesting stability of the resistance. Comparison of the pyrrolnitrin-resistant generations and their sensitive parental isolates for mycelial growth, sporulation and aggressiveness on plant tissues revealed that the high level of resistance to pyrrolnitrin resulted in a high fitness cost. Mycelial growth was reduced between 1.7 to 3.6 times and sporulation reduced 3.8 to 6.6 times that of sensitive parental isolates. Similarly, aggressiveness was 7 to 10 and 3 to 10 times lower for resistant isolates on tomato and apple, respectively. This study provides evidence that a fungal plant pathogen is able to gradually build up resistance to an antibiotic produced by a biocontrol agent.
Fichier principal
Vignette du fichier
35455_20101026022441318_1.pdf (525.67 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02668915 , version 1 (31-05-2020)

Licence

Paternité - Partage selon les Conditions Initiales

Identifiants

Citer

Sakhr Ajouz, Philippe C. Nicot, Marc Bardin. Adaptation to pyrrolnitrin in Botrytis cinerea and cost of resistance. Plant Pathology, 2010, 59 (3), pp.556-566. ⟨10.1111/j.1365-3059.2009.02230.x⟩. ⟨hal-02668915⟩
24 Consultations
53 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More