In a three-year field experiment in Toulouse (in Southwest France), two indicators of plant nitrogen (N) status were compared on five durum wheat cultivars: the normalized SPAD index and the nitrogen nutrition index (NNI). SPAD value is a non-destructive measurement of chlorophyll content from the last expanded leaf. The normalized SPAD index is expressed relative to SPAD reading on a fully fertilized crop. The NNI is calculated from the crop biomass and total plant N content using a universal N-dilution curve for wheat. The normalized SPAD index and NNI were closely related irrespective of year, cultivar, and growth stage. When N was a limiting factor, the SPAD index measured at an thesis predicted grain yield and protein content accurately. Unlike NNI, SPAD index cannot be used to predict these variables when wheat is over-fertilized.