23Na NMR study of fibrobacter succinogenes S85 : Comparison of three chemical shift raegents and caculation of sodium concentration using ionophores - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Analytical Biochemistry Année : 2002

23Na NMR study of fibrobacter succinogenes S85 : Comparison of three chemical shift raegents and caculation of sodium concentration using ionophores

A.M. Delort
Evelyne Forano

Résumé

In order to measure intracellular sodium concentrations in resting cells of Fibrobacter succinogenes S85 by 23Na NMR spectrometry, two methodological aspects were studied. First, three different shift reagents (Dy(PPPi)7−2, Tm(DOTP)5−, and Dy(TTHA)3−) were tested for their ability to separate internal and external 23Na NMR resonances. Their toxicity toward F. succinogenes cells was evaluated by in vivo13C NMR experiments. Tm(DOTP)5− was found to be the most efficient shift reagent while being nontoxic. Second, a new methodology was developed to calculate intracellular sodium concentration in F. succinogenes by using ionophores. This approach avoided the problem of intracellular volume measurement and that of sodium visibility determination.

Domaines

Biotechnologies

Dates et versions

hal-02672171 , version 1 (31-05-2020)

Identifiants

Citer

A.M. Delort, Gérard Gaudet, Evelyne Forano. 23Na NMR study of fibrobacter succinogenes S85 : Comparison of three chemical shift raegents and caculation of sodium concentration using ionophores. Analytical Biochemistry, 2002, 306 (2), pp.171-180. ⟨10.1006/abio.2002.5685⟩. ⟨hal-02672171⟩
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