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23Na NMR study of fibrobacter succinogenes S85 : Comparison of three chemical shift raegents and caculation of sodium concentration using ionophores

Abstract : In order to measure intracellular sodium concentrations in resting cells of Fibrobacter succinogenes S85 by 23Na NMR spectrometry, two methodological aspects were studied. First, three different shift reagents (Dy(PPPi)7−2, Tm(DOTP)5−, and Dy(TTHA)3−) were tested for their ability to separate internal and external 23Na NMR resonances. Their toxicity toward F. succinogenes cells was evaluated by in vivo13C NMR experiments. Tm(DOTP)5− was found to be the most efficient shift reagent while being nontoxic. Second, a new methodology was developed to calculate intracellular sodium concentration in F. succinogenes by using ionophores. This approach avoided the problem of intracellular volume measurement and that of sodium visibility determination.
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https://hal.inrae.fr/hal-02672171
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Submitted on : Sunday, May 31, 2020 - 4:03:41 PM
Last modification on : Tuesday, June 22, 2021 - 12:02:02 PM

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A.M. Delort, Gérard Gaudet, Evelyne Forano. 23Na NMR study of fibrobacter succinogenes S85 : Comparison of three chemical shift raegents and caculation of sodium concentration using ionophores. Analytical Biochemistry, Elsevier Masson, 2002, 306 (2), pp.171-180. ⟨10.1006/abio.2002.5685⟩. ⟨hal-02672171⟩

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