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Detailed physico-chemical characterisation of the 2S storage protein from rape (Brassica Napus L.)

Abstract : Chromatographic, chemical, and spectroscopic techniques were used to characterize the physicochemical properties of napin purified by preparative chromatography. The molar extinction coefficient was determined (ε=0.56), and static and dynamic light scattering measurements enabled the average molecular weight (Mw ) 13919), the second virial coefficient (A2 ) 23.95 χ 10-5 mol cm3 g-2), and the hydrodynamic radius (RH ) 1.98 nm) to be determined. No conformational changes were observed by fluorescence and circular dichroism measurements in different buffers at pH 3, 4.6, 7, and 12, confirming the high pH stability of this protein. From MALDI-TOF analysis and after enzymatic digestion, it was found that this purified sample, extracted from the rapeseed variety Express, contained mainly isoform 2SS3_BRANA.
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https://hal.inrae.fr/hal-02677569
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Submitted on : Sunday, May 31, 2020 - 7:32:46 PM
Last modification on : Wednesday, October 20, 2021 - 3:19:18 AM

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  • HAL Id : hal-02677569, version 1
  • PRODINRA : 282822

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Isabelle Schmidt, Denis Renard, David Rondeau, Pascal Richomme, Yves Popineau, et al.. Detailed physico-chemical characterisation of the 2S storage protein from rape (Brassica Napus L.). Journal of Agricultural and Food Chemistry, American Chemical Society, 2004, 52, pp.5995-6001. ⟨hal-02677569⟩

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