Screening of glutamate decarboxylase activity and bile salt resistance of human asymptomic carriage, clinical, food, and environmental isolates of Listeria monocytogenes - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue International Journal of Food Microbiology Année : 2004

Screening of glutamate decarboxylase activity and bile salt resistance of human asymptomic carriage, clinical, food, and environmental isolates of Listeria monocytogenes

Résumé

Following consumption, stomach acidity is the first major barrier encountered by the food-borne pathogen Listeria monocytogenes. Analysis of low pH sensitivity and glutamate decarboxylase (GAD) acid resistance system of 14 isolates of L. monocytogenes carried asymptomatically by humans showed that levels of GAD activity was subjected to strain variation. Similar variations were observed for strains responsible of 18 listeriosis, whereas in comparison, 13 strains isolated from food and from food-processing plant environment showed lower GAD activity. Following survival of the stomach barrier, L. monocytogenes also has to resist to bile salts encountered in the small intestine. Analysis revealed that all strains tested were able to grow in presence of bile salts with concentrations as high as those encountered in the small intestine and had the previously identified Bile Salt Hydrolase (BSH) activity. Strain variation was observed but there was no relationship between the origin of the strains and the ability to degrade bile salts.
Fichier principal
Vignette du fichier
2004 Olier et al GAD BSH.pdf (659.45 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Licence : CC BY NC ND - Paternité - Pas d'utilisation commerciale - Pas de modification

Dates et versions

hal-02677729 , version 1 (12-01-2022)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

  • HAL Id : hal-02677729 , version 1
  • PRODINRA : 77621

Citer

Maïwenn Olier, Sandrine Rousseaux, P. Piveteau, J.P. Lemaître, André Rousset, et al.. Screening of glutamate decarboxylase activity and bile salt resistance of human asymptomic carriage, clinical, food, and environmental isolates of Listeria monocytogenes. International Journal of Food Microbiology, 2004, 93 (1), pp.87-99. ⟨hal-02677729⟩
17 Consultations
6 Téléchargements

Partager

Gmail Facebook X LinkedIn More