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Oligosaccharide synthesis in fibrobacter succinogenes S85 and its modulation by the substrate

Abstract : In this article we compared the metabolism of phosphorylated and unphosphorylated oligosaccharides (cellodextrins and maltodextrins) in Fibrobacter succinogenes S85 resting cells incubated with the following substrates: glucose; cellobiose; a mixture of glucose and cellobiose; and cellulose. Intracellular and extracellular media were analysed by H-1-NMR and by TLC. The first important finding is that no cellodextrins were found to accumulate in the extracellular media of cells, regardless of the substrate; this contrasts to what is generally reported in the literature. The second finding of this work is that maltodextrins of degree of polymerization > 2 are synthesized regardless of the substrate, and can be used by the bacteria. Maltotriose plays a key role in this metabolism of maltodextrin. Maltodextrin-1-phosphate was detected in all the incubations, and a new metabolite, corresponding to a phosphorylated glucose derivative, was produced in the extracellular medium when cells were incubated with cellulose. The accumulation of these phosphorylated sugars increased with the degree of polymerization of the substrate.
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Submitted on : Sunday, May 31, 2020 - 8:26:49 PM
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R. Nouaille, M. Matulová, A.M. Delort, Evelyne Forano. Oligosaccharide synthesis in fibrobacter succinogenes S85 and its modulation by the substrate. FEBS Letters, Wiley, 2005, 272 (10), pp.2416-2427. ⟨10.1111/j.1742-4658.2005.04662.x⟩. ⟨hal-02678426⟩



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