Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Resistance phenotypes of transgenic tobacco plants expressing different cucumber mosaic virus (CMV) coat protein genes

Abstract : Tobacco plants expressing a transgene encoding the coat protein (CP) of a subgroup I strain of cucumber mosaic cucumovirus (CMV), I17F, were not resistant to strains of either subgroup I or II. In contrast, the expression of the CP of a subgroup II strain, R, conferred substantial resistance, but only towards strains of the same subgroup. When protection was observed, the levels of resistance were similar when plants were inoculated with either virions or viral RNA, but resistance was more effective when plants were inoculated with viruliferous aphids. Resistance was not dependent on inoculum strength and was expressed as a recovery phenotype not yet described for plants expressing a CMV CP gene. Recovery could be observed either early in infection (less than one week after inoculation) or later (4 to 5 weeks after inoculation). In plants showing early recovery, mild symptoms were observed on the inoculated leaves, and in some cases symptoms developed on certain lower systemically infected leaves, but the upper leaves were symptomless and virus-free. Late recovery corresponded to the absence of both symptoms and virus in the upper leaves of plants that were previously fully infected. Northern blot analyses of resistant plants suggested that a gene silencing mechanism was not involved in the resistance observed.
Liste complète des métadonnées

https://hal.inrae.fr/hal-02680325
Déposant : Migration Prodinra <>
Soumis le : dimanche 31 mai 2020 - 22:13:55
Dernière modification le : vendredi 12 juin 2020 - 10:43:26

Lien texte intégral

Identifiants

Collections

Citation

Mireille Jacquemond, P.Y. Teycheney, Isabelle Carrère, Jesus Navas Castillo, Mark Tepfer. Resistance phenotypes of transgenic tobacco plants expressing different cucumber mosaic virus (CMV) coat protein genes. Molecular Breeding, Springer Verlag, 2001, 8, pp.85-94. ⟨10.1023/A:1011918518091⟩. ⟨hal-02680325⟩

Partager

Métriques

Consultations de la notice

7