Skip to Main content Skip to Navigation
Journal articles

Mice immunization with live lactococci displaying a surface anchored HPV-16 E7 oncoprotein.

Abstract : E7 oncoprotein of human papillomavirus-16 (HPV-16) is constitutively produced in cervical cancer (CxCa) and is a good candidate for the design of therapeutic vaccines. In this work, the nisin-controlled expression system was used to display the E7 protein at the cell surface of the food-grade Gram-positive bacterium Lactococcus lactis. An efficient cell wall anchoring of E7 was obtained. Intranasal administration of these recombinant lactococci in mice induced an HPV-16 E7-specific immune response. This is the first report of E7 cell wall anchoring in L. lactis and represents one more step towards the use of live food-grade bacteria to fight against CxCa.
Complete list of metadata

https://hal.inrae.fr/hal-02682491
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Monday, June 1, 2020 - 1:12:55 AM
Last modification on : Thursday, September 23, 2021 - 4:28:03 PM

Links full text

Identifiers

Collections

Citation

Naima G Cortes-Perez, Luis Bermudez Humaran, Yves Le Loir, Cristina Rodríguez-Padilla, Alexandra Gruss, et al.. Mice immunization with live lactococci displaying a surface anchored HPV-16 E7 oncoprotein.. FEMS Microbiology Letters, Wiley-Blackwell, 2003, 229, pp.37-42. ⟨10.1016/S0378-1097(03)00778-X⟩. ⟨hal-02682491⟩

Share

Metrics

Record views

25