Mice immunization with live<em> lactococci</em> displaying a surface anchored HPV-16 E7 oncoprotein. - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue FEMS Microbiology Letters Année : 2003

Mice immunization with live lactococci displaying a surface anchored HPV-16 E7 oncoprotein.

Résumé

E7 oncoprotein of human papillomavirus-16 (HPV-16) is constitutively produced in cervical cancer (CxCa) and is a good candidate for the design of therapeutic vaccines. In this work, the nisin-controlled expression system was used to display the E7 protein at the cell surface of the food-grade Gram-positive bacterium Lactococcus lactis. An efficient cell wall anchoring of E7 was obtained. Intranasal administration of these recombinant lactococci in mice induced an HPV-16 E7-specific immune response. This is the first report of E7 cell wall anchoring in L. lactis and represents one more step towards the use of live food-grade bacteria to fight against CxCa.

Dates et versions

hal-02682491 , version 1 (01-06-2020)

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Citer

Naima G Cortes-Perez, Luis Bermudez Humaran, Yves Le Loir, Cristina Rodríguez-Padilla, Alexandra A. Gruss, et al.. Mice immunization with live lactococci displaying a surface anchored HPV-16 E7 oncoprotein.. FEMS Microbiology Letters, 2003, 229, pp.37-42. ⟨10.1016/S0378-1097(03)00778-X⟩. ⟨hal-02682491⟩

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