Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Gurke and pasticcino3 mutants affected in embryo development are impaired in acetyl-CoA carboxylase

Abstract : Normal embryo development is required for correct seedling formation. The Arabidopsis gurke and pasticcino3 mutants were isolated from different developmental screens and the corresponding embryos exhibit severe defects in their apical region, affecting bilateral symmetry. We have recently identified lethal acc1 mutants affected in acetyl-CoA carboxylase 1 (ACCase 1) that display a similar embryo phenotype. A series of crosses showed that gk and pas3 are allelic to acc1 mutants, and direct sequencing of the ACC1 gene revealed point mutations in these new alleles. The isolation of leaky acc1 alleles demonstrated that ACCase I is essential for correct plant development and that mutations in ACCase affect cellular division in plants, as is the case in yeast. Interestingly, significant metabolic complementation of the mutant phenotype was obtained by exogenous supply of malonate, suggesting that the lack of cytosolic malonyl-CoA is likely to be the initial factor leading to abnormal development in the acc1 mutants.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.inrae.fr/hal-02682755
Déposant : Migration Prodinra <>
Soumis le : lundi 1 juin 2020 - 01:45:20
Dernière modification le : lundi 9 novembre 2020 - 17:44:05

Lien texte intégral

Identifiants

Collections

Citation

Sebastien Baud, Yannick Bellec, C. Miquel, Catherine Bellini, Michel Caboche, et al.. Gurke and pasticcino3 mutants affected in embryo development are impaired in acetyl-CoA carboxylase. EMBO Reports, EMBO Press, 2004, 5 (5), pp.515-520. ⟨10.1038/sj.embor.7400124⟩. ⟨hal-02682755⟩

Partager

Métriques

Consultations de la notice

8