Xylanases and carboxymethylcellulases of the rumen protozoa Polyplastron multivesiculatum, Eudiplodinium maggii and Entodinium sp - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue FEMS Microbiology Letters Année : 2005

Xylanases and carboxymethylcellulases of the rumen protozoa Polyplastron multivesiculatum, Eudiplodinium maggii and Entodinium sp

Résumé

Endoglucanase and xylanase activities of three rumen protozoa, Polyplastron multivesiculatum, Eudiplodinium maggii, and Entodinium sp. were compared qualitatively by zymograms and quantitatively by measuring specific activities against different polysaccharides. A set of carboxymethylcellulases and xylanases was produced by the large ciliates whereas no band of activity was observed for Entodinium sp. in zymograms. Specific activity of endoglucanases from P. multivesiculatum (1.3 mumol mg prot (-1) min(-1)) was twice that of E. maggii, whereas xylanase specific activity (4.5 mumol mg prot(-1) min(-1)) was only half. Very weak activities were observed for Entodinium sp. A new xylanase gene, xynIID, from P. multivesiculatum was reported and its gene product compared to 33 other family I I xylanases. Phylogenetic analysis showed that xylanase sequences from rumen protozoa are closely related to those of bacteria. (C) 2005 Federation of European Microbiological Societies.

Dates et versions

hal-02683234 , version 1 (01-06-2020)

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Christel Béra-Maillet, Estelle Devillard, Magalie Cezette, J Pierre Jouany, Evelyne Forano. Xylanases and carboxymethylcellulases of the rumen protozoa Polyplastron multivesiculatum, Eudiplodinium maggii and Entodinium sp. FEMS Microbiology Letters, 2005, 244 (1), pp.149-156. ⟨10.1016/j.femsle.2005.01.035⟩. ⟨hal-02683234⟩
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