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Interaction of the Grb7 adapter protein with Rnd1, a new member of the Rho family

Abstract : Grb7 is a member of a family of molecular adapters which are able to contribute positively but also negatively to signal transduction and whose precise roles remain obscure. Rnd1 is a member of the Rho family, but, as opposed to usual GTPases, it is constitutively bound to GTP. We show here that Rnd1 and Grb7 interact, in two-hybrid assays, in vitro, and in pull-down experiments performed with SK-BR3, a breast cancer cell line that overexpresses Grb7. This interaction involves switch II loop of Rnd1, a region crucial for guanine nucleotide exchange in all GTPases, and a Grb7 SH2 domain, a region crucial for Grb7 interaction with several activated receptors. The contribution of the interaction between Rnd1 and Grb7 to their respective functions and properties is discussed.
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Béatrice Vayssière, Gérard Zalcman, Yannick Mahe, Gladys Mirey, Tanja Ligensa, et al.. Interaction of the Grb7 adapter protein with Rnd1, a new member of the Rho family. FEBS Letters, Wiley, 2000, 467 (1), pp.91-96. ⟨10.1016/S0014-5793(99)01530-6⟩. ⟨hal-02695354⟩



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