Skip to Main content Skip to Navigation
Journal articles

Construction of full-length cDNA clones of lettuce mosaic virus (LMV) and the effects of intron-insertion on their viability in Escherichia coli and on their infectivity to plants

Abstract :  A full length cDNA copy of the genomic RNA of lettuce mosaic virus (LMV) was constructed under the control of an enhanced CaMV 35S promoter and of the NOS terminator. This construct was found infectious when inoculated to lettuce plants. The intron II of the bean nitrite reductase gene was engineered into the LMV FL cDNA in order to relieve possible deleterious effects of viral sequences to Escherichia coli cells and to evaluate the effects of the presence of the intron on the FL cDNA infectivity. The intron-less FL cDNA was found to be as stable as its intron-containing counterpart in E. coli. Sequence analysis of progeny RNA derived from plants inoculated with the intron-containing FL cDNA demonstrated that the inserted intron was perfectly spliced out. The symptoms induced in lettuce by either the intron-less or the intron-containing constructs were identical to those caused by the wild-type virus. However a slight delay in the establishment of infection in lettuce and a more obvious lag in Nicotiana benthamiana were observed with the intron-containing FL cDNA.
Complete list of metadata

https://hal.inrae.fr/hal-02698140
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Monday, June 1, 2020 - 11:50:49 AM
Last modification on : Wednesday, August 25, 2021 - 10:52:07 AM

Links full text

Identifiers

Collections

Citation

S.J. Yang, Frederic Revers, Sylvie Souche, Hervé Lot, Olivier Le Gall, et al.. Construction of full-length cDNA clones of lettuce mosaic virus (LMV) and the effects of intron-insertion on their viability in Escherichia coli and on their infectivity to plants. Archives of Virology, Springer Verlag, 1998, 143 (12), pp.2443-2451. ⟨10.1007/s007050050474⟩. ⟨hal-02698140⟩

Share

Metrics

Record views

32