Diversity of <em>Paenibacillus polymyxa</em> populations in the rhizosphereof wheat (<em>Triticum durum</em>) in Algerian soils - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue European Journal of Soil Biology Année : 2000

Diversity of Paenibacillus polymyxa populations in the rhizosphereof wheat (Triticum durum) in Algerian soils

Souad Guemouri-Athmani
  • Fonction : Auteur
Odile Berge
Muriel Bourrain
  • Fonction : Auteur
Patrick Mavingui
Jean M. Thiéry
  • Fonction : Auteur
Tej Bhatnagar
  • Fonction : Auteur
Thierry Heulin
  • Fonction : Auteur

Résumé

The diversity of Paenibacillus polymyxa populations associated with the rhizosphere of durum wheat was investigated in Algerian soils sampled in regions where wheat had been cultivated for 5 and 26 years (Hamiz, H5 and Z26), 70 years (Algiers, D70), and more than 2 000 years (Tiaret, T2000 and K2000). A total of 111 strains were isolated by immunotrapping and identified as P. polymyxa using an API50CHB kit and restriction analysis of the amplified 16S rDNA gene. The phenotypic characteristics of the P. polymyxa populations were compared and the strains found not to cluster according to their origin. The longer the history of wheat cultivation, the lower the phenotypic diversity and the higher the frequency of nitrogen-fixing strains. Population genetic diversity, evaluated by ERIC-PCR, showed the same trends as phenotypic diversity. The distribution of ERIC genotypes among the different populations studied were compared using Pearson’s Chi-squared test. The strains isolated in D70, Z26, and H5 derived from soil populations sharing the same genetic structure, but those isolated in T2000 and K2000 each stemmed from a population with a specific genetic structure. These data suggest that the genetic structure of P. polymyxa populations has been affected by long-term wheat cultivation.

Dates et versions

hal-02699025 , version 1 (01-06-2020)

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Citer

Souad Guemouri-Athmani, Odile Berge, Muriel Bourrain, Patrick Mavingui, Jean M. Thiéry, et al.. Diversity of Paenibacillus polymyxa populations in the rhizosphereof wheat (Triticum durum) in Algerian soils. European Journal of Soil Biology, 2000, 36, pp.149-159. ⟨10.1016/S1164-5563(00)01056-6⟩. ⟨hal-02699025⟩

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