A specific PCR to differentiate between gE negative vaccine and wildtype bovine herpesvirus type 1 strains - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Veterinary Microbiology Year : 1999

A specific PCR to differentiate between gE negative vaccine and wildtype bovine herpesvirus type 1 strains

Eric Baranowski
M Lemaire
  • Function : Author

Abstract

In the context of infectious bovine rhinotracheitis (IBR) control programmes using glycoprotein E (gE) deleted marker vaccines, a PCR assay was developed to allow the genotypic differentiation between wildtype bovine herpesvirus type 1 (BoHV-1) and gE negative strains. This assay is based on the PCR amplification of a 281 bp DNA fragment within the gE gene. The specificity of the amplification was confirmed by restriction endonuclease analysis and nucleotide sequencing of the PCR product. Its ability to determine the gE genotype of BoHV-1 strains was demonstrated on isolates coming from 20 experimental calves infected with four different BoHV-1 strains. This PCR assay may be a useful tool for monitoring the spread of live marker vaccine and the gE genotype of viral field isolates.

Dates and versions

hal-02699051 , version 1 (01-06-2020)

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Frédéric Schynts, Eric Baranowski, M Lemaire, Etienne Thiry. A specific PCR to differentiate between gE negative vaccine and wildtype bovine herpesvirus type 1 strains. Veterinary Microbiology, 1999, 66 (3), pp.187-195. ⟨10.1016/S0378-1135(99)00008-5⟩. ⟨hal-02699051⟩

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