Somatic embryogenesis in cotyledons of Picea abies is enhanced by and adventitious bud-inducing treatment
Amplification de l'embryogenese somatique dans les cotyledons de l'Epicea commun par un traitement inductif des bourgeons adventifs
Résumé
Somatic embryos were obtained from young, isolated cotyledons of Picea abies. A bud-inductive treatment for one week before transfer to a callus induction medium, resulted in a tenfold increase in the percentage of cotyledons in which embryonic tissues could be induced. The degree of expression of somatic embryogeny depends on whether cotyledons are first subjected to a callus-inducing medium or first exposed to a cytokinin treatment followed by a callus-inducing medium. It is hypothesized that two populations of cells exist, both with the inherent potential of becoming embryogenic after treatment with plant growth regulators.
Domaines
Biologie végétale
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