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Northern blot analysis of the Na+, K+-ATPase alpha subunit in salmonids

Abstract : A 450-bp long fragment of the gene encoding the Na+, K+-ATPase alpha-subunit was PCR (polymerase chain reaction)-amplified from the rainbow trout (Oncorhynchus mykiss) genome. The protein coding sequence of the cloned PCR product shared 85 and 73% identity with Na+, K+-ATPase alpha-subunit-encoding sequences of the rat (alpha(2)-isoform) and the teleost species of the white sucker (Carostomus commersoni), respectively. Northern blot analysis demonstrated that the fragment hybridized to a major transcript of about 3.7 kb in several salmonid and non-salmonid species. The observed changes in Na+, K+-ATPase mRNA levels in osmoregulatory organs of seawater- and freshwater-adapted fish further confirm the specificity of the probe.
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Gunn Kisen, Claudiane Gallais, Benoît Auperin, H. Klungland, Olivier Sandra, et al.. Northern blot analysis of the Na+, K+-ATPase alpha subunit in salmonids. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, Elsevier, 1994, 107B (2), pp.255-259. ⟨10.1016/0305-0491(94)90047-7⟩. ⟨hal-02715918⟩

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