, Sa progression a pu être observée par la technique classique de visualisation des tubes polliniques par le Bleu d'Aniline (méthode ABF) (fig. 5 et 7). L'utilisation du microscope électronique à balayage couplé à un système cryogénique EMSCOPE SP, Nous avons examiné les principales étapes de la reproduction sexuée chez le Peuplier noir (Populus nigra), 2000.

, Mot.s clés : Pollen, stigmate, ovule, Populus

D. Umas, C. , and R. B. , Callose and determination of pistil viability and incompatibility, Theor. Appl. Genet, vol.67, pp.1-10, 1983.

G. Aget, M. Aid-c, and R. B. Umas-c, a Pollen-Pistil interactions in interspecific crosses of Populus (Section Aigeiros & L f tfff) : pollen adhesion, hydratation and callose responscs, J. Cell Sci, vol.72, pp.173-184, 1984.

G. Aget, M. V-illar-m, . Umas-c, and R. E. Emoine-m..-Ï-ehstër-du-c, Poplar improvment : new stratégies currently in progress in France, Proc. of the XVllth Session of the international Poplar commission, pp.25-30, 1984.

H. Am-m-t-on-d, Inter,!ectional incompatibility in Populus, 1976.

H. Eslop--h-arr-tson, J. , H. Eslop--h-arrison, and Y. , Evaluation of pollen viability by enzymatically induced fluorescence, intracellular hydrolysis of fluorescein diacetate, Stain Technol, vol.45, pp.115-120, 1970.

H. , H. Eslop--h-arrison, and Y. , Pollen-tube chemotropism : fact or delusion ? In : Biology of reproduction and cell motility in plants and animal, pp.169-174, 1986.

S. F. Te-tt-ler-r and A. A. Ger-a, Mentor effect in pollen interactions, pp.609-623, 1984.

S. F. Te-tt-ler-r, S. Oster-r, and . Teenackers-v, Interspeeific crossability studies in Poplars, Theor. Appl. Genet, vol.58, pp.273-282, 1980.

V. Illar and M. , Incompatibilité interspécifique chez Populus : approches physiologique et biochimique, 1987.

V. Illar, M. Aget-m, and D. Umas-c., Sexual reproduction biology in Populus : compatibility and incompatibility, Biotechnology and ecology of pollen, pp.514-517, 1986.

W. R. Illing-r and P. D. Ryor-l, Interspecific hybridization in Poplars, Theor. Appl. Genet, vol.47, pp.141-151, 1976.