Emphasis given in France to reforestation with mazzard (Prunus avium L.) and difficulties in applying classical means of vegetative propagation (cuttings or suckerings) have led to consider in vitro cultures to propagate mature trees selected in stands. Cultures were established with buds collected either on crown shoots or on young root suckers. Different factors were studied : macroelement solutions, growth regulators, light and temperature. The basic substrate is a slightly modified M.S. salt medium (half-concentration of NH4NO3) with 2 p. 100 sucrose and 7 p. 100 agar. Whole plants are obtained after a series of stages with a particular medium for each of them. 1. Initiation of the culture after a strong sterilization with mercuric chloride (2 x 10-2 M). 2. Multiple bud production with BA 4 x 10-6 M and IBA, 5 x 10-7 M and GA 3 x 10-7 M. 3. Shoot elongation with a high concentration of GA : 3 x 10-5 M. 4. Rooting on a low macroelements concentrated medium (dilution by 1/5 of basic medium) after short dipping in a IBA aqueous solution (2.5 to 5 x 10-3 M). 5. Transfert to soil with greenhouse acclimation. 70 trees are presently under culture. Two years after initiating this work, 20 clones are already transplanted in the nursery.