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Poster communications

Évaluation de l’innocuité et des effets immunostimulants d’extraits végétaux sur lignées cellulaires de poulet

Abstract : Maintaining optimal conditions of welfare and animal health is a daily concern in livestock breeding. The use of plant extracts in poultry feed seems an interesting approach to reinforce the animals’ natural defenses. However, this approach is limited by the lack of robust references and solid methodological bases. Our objective was to develop and test a simple, reliable and repeatable in vitro method to determine cytotoxic and immunostimulant capacities of plant extracts. A bibliographic study helped identifying 4 plant extracts: Astragalus, Echinacea, Ginseng and Melissa. A metabolic activity test (MTT) was carried out on three chicken cell lines, representative of the respiratory (CLEC213), hepatic (LMH) and immune (HD11 macrophages) functions. Immunostimulation was studied in macrophages by assessing the production of the pro-oxidant and antimicrobial molecule nitric oxide (NO) and the activation of the pro-inflammatory NFκB pathway. We have shown that Astragalus, Echinacea, Ginseng and Melissa had little or no cytotoxic effects. Melissa and Ginseng have the ability to significantly activate cell metabolism (50-100%), to induce the production of NO (10 to 20-fold) or the activation of the NFκB pathway (5 to 18-fold), thus suggesting immunostimulatory activities. In conclusion, extracts’ preparation steps, cell type’s choice and cell culture conditions allowed us to define a relevant methodology to assess cytotoxic and immunostimulant properties of the extracts. The Melissa and Ginseng extracts appear to be non-cytotoxic candidates, capable of stimulating the natural defenses of poultry.
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Poster communications
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https://hal.inrae.fr/hal-02734430
Contributor : Migration Prodinra <>
Submitted on : Tuesday, June 2, 2020 - 1:58:20 PM
Last modification on : Thursday, August 5, 2021 - 3:55:03 AM

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  • HAL Id : hal-02734430, version 1
  • PRODINRA : 465324

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Laurine Allimonnier, Denis Bellenot, Benjamin Lemaire, Laurence Guilloteau, Angélique Travel, et al.. Évaluation de l’innocuité et des effets immunostimulants d’extraits végétaux sur lignées cellulaires de poulet. 13. Journées de la Recherche Avicole et Palmipèdes à Foie Gras, Mar 2019, Tours, France. ITAVI - Institut Technique de l'Aviculture, 929 p., 2019, 13èmes Journées de la Recherche Avicole et Palmipèdes à Foie Gras. ⟨hal-02734430⟩

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