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The Ca2+ regime at fertilization is determined by the formulation of the culture media

Abstract : In mammals, fertilization triggers a series of Ca2+ oscillations the frequency, number and duration of which are highly variable. Several reports demonstrate that the culture media used at fertilization affects the developmental potential and may have postnatal consequences. Given the considerable use of in-vitro fertilization technologies in human, the elucidation of the programming role played by the formulation of the culture media remains a critical challenge. In order to assess the functional impact of the culture media better, we questioned whether the number of Ca2+ oscillation, the time period between oscillations and the total duration of the Ca2+ regime at fertilization in the presence of different commercial culture media makes it possible to establish a quantitative connection between media formulation and the developmental consequences. We measured the Ca2+ signal induced by ICSI in the presence of M16, KSOM, Vitrolife and Cook medium in the mouse. The results show that the parameters of Ca2+ signaling at fertilization are highly determined by the formulation of the culture media. KOM triggers twice as much Ca2+ oscillations than M16 and M16 has twice as much Ca2+ oscillations than Vitrolife and Cook media.
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https://hal.inrae.fr/hal-02741120
Contributor : Migration Prodinra <>
Submitted on : Wednesday, June 3, 2020 - 12:37:27 AM
Last modification on : Thursday, August 5, 2021 - 3:45:03 AM

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  • HAL Id : hal-02741120, version 1
  • PRODINRA : 354355

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Jean-Pierre Ozil, Thierry Sainte-Beuve, Bernadette Banrezes. The Ca2+ regime at fertilization is determined by the formulation of the culture media. Epiconcept Workshop 2016 "Epigenetics and Periconception Environmenent", European Cooperation in Science and Technology (COST). BEL., May 2016, Velingrad, Bulgaria. 41 p. ⟨hal-02741120⟩

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