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Impact on epigenetic machinery genes expression and on foeto-placental development of preconceptional maternal weight loss

Abstract : Whether the currently recommended preconceptional weight loss (WL) for obese women is beneficial/detrimental for fetoplacental development remains poorly explored and even less on the long term. Obesity, even if corrected before conception, and the WL processes could be "memorized" via epigenetic mechanisms perturbing expression of key developmental genes leading to altered fetal programming. Our aim was to investigate the effects of maternal WL alter diet-induced obesity on feto­placental development and on gene expression in male and female mice offspring. During the preconception period, female C57BI/6J mice were led a high-fat diet (HFD) for two months, leading to obesity and then a control die! (CD) for two months, leading to a complete WL. ln parallel, obese control group (OC) was led a HFD and lean control group (LC) a CD throughout the preconceptional period and gestation. At embryonic day 18 (E18.5), OC fetuses presented intrauterine growth retardation (IUGR), while WL fetuses, despite maternal weight normalization before conception, showed a significantly decreased weight at term which was intermediate between OC and LC fetuses. Placental weight was not affected by diet. Gene expression of 60 epigenetic machinery and 30 key developmental genes was analysed in the placental labyrinth and fetal liver using RTq-PCR Taqman custom array card. Expression of several epigenetic machinery genes was altered in OC fetuses. ln WL fetuses the level of expression of some genes was restored to the level of LC fetuses while ether genes showed similar profiles as in OC fetuses. Thus correction of maternal obesity by nutritional intervention in the preconceptional period only partially protects the offspring from IUGR. We show that modulation of the histone acetylation-deacetylation switch could play a role in fetal programming. Further studies at the genome-wide level will elucidate the epigenetic targets and mechanisms of placental/fetal programming triggered by maternal ponderal trajectories.
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https://hal.inrae.fr/hal-02741132
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Submitted on : Wednesday, June 3, 2020 - 12:39:26 AM
Last modification on : Thursday, August 5, 2021 - 3:51:16 AM

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  • HAL Id : hal-02741132, version 1
  • PRODINRA : 277159

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Polina Panchenko, Luc Jouneau, Mélanie Jouin, Marion Lemaire, Claudine Junien, et al.. Impact on epigenetic machinery genes expression and on foeto-placental development of preconceptional maternal weight loss. 2. Colloque de la SF-DOHaD, Origine Développementale de la santé et des maladies et épigénétique, Société Francophone pour la Recherche et l'Education sur les Origines Développementales, Environnementales et Epigénétiques de la Santé et des Maladies (SF-DOHAD). FRA., Nov 2014, Nantes, France. 57 p. ⟨hal-02741132⟩

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