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Communication Dans Un Congrès Année : 2015

Stress disrupts the intestinal mucus barrier in rats via mucin O-glycosylation shift: prevention by probiotic treatment

Résumé

Background: Despite evidence of intestinal epithelial barrier impairment and visceral hypersensitivity in IBS patients and IBS-like models, structural and physical changes in the mucus layer in this condition remain poorly understood. Aim: Using a water avoidance stress (WAS) model, we aimed at evaluating in ileum and colon whether (i) WAS modified gut permeability, visceral sensitivity, mucin expression, biochemical structure of O-glycans and related mucus physical properties, (ii)L. farcimin- is treatment prevented these alterations. Methods: Wistar rats received orally L. farciminis (an adhesion marker) or vehicle for 14 days. At day 10, they were submitted either to sham or 4-day WAS. Intestinal paracellular permeability and visceral sensi- tivity were measured in vivo . The number of goblet cells and Muc2 expression were evaluated by histology and immunohistochemistry, respectively. Mucosal adhesion of L. farciminis was determined ex situ . The mucin O-glycosylation profile was obtained by mass spectrom- etry. Surface imaging of intestinal mucus was performed at nanoscale by Atomic Force Microscopy. Results: WAS induced gut hyperpermeability and vis- ceral hypersensitivity but did not modify either the number of intestinal goblet cells or Muc2 expression
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Dates et versions

hal-02742282 , version 1 (03-06-2020)

Identifiants

  • HAL Id : hal-02742282 , version 1
  • PRODINRA : 343257
  • WOS : 000356078300029

Citer

Stéphanie da Silva, Catherine Robbe Masselot, Afifa Ait-Belgnaoui, Allesandro Mancuso, Myriam Mercade Loubière, et al.. Stress disrupts the intestinal mucus barrier in rats via mucin O-glycosylation shift: prevention by probiotic treatment. 26. Meeting of European Intestinal Transport Group (EITG), European Intestinal Transport Group (EITG). INT., Oct 2014, Marstrand, Sweden. 2 p. ⟨hal-02742282⟩
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