Polycyclic aromatic hydrocarbons (PAH) emission in the environment is constantly increasing with human activity. The goal of this study was to assess long term consequences of fish early exposure to PAH on behaviour. Eggs were collected from AB zebrafish strain and were placed at 4 hours post fertilization (hpf) until 96 hpf in a 3 cm diameter Petri dish containing 3 g reference sediment either plain (control) or spiked with a mixture of Benzo[a]pyrene, Pyrene and Phenanthrene at environmental concentrations based on values measured in the Seine Estuary (France), a highly polluted site. Thereafter, larvae were transferred in clean water and raised until adulthood. Behavioural tests such as locomotion during 24-hrs, sudden dark change challenge and T-maze exploration were performed at adults’ stage (F0) and sudden dark change on larvae produced by F0 adults. In adults, during night or dark period, contaminated fish were significantly less active than control fish. A similar observation was made for contaminated F0 in T-maze safe area. Contaminated F1 larvae were more active when light was on than control and less active when the light was off. This study indicates that contamination of zebrafish using environmentally relevant concentration during the very first stage of development with a PAH mixture of 3 molecules induced late behavioural effects measured at the adult stage. Further, effect was transmitted to the next generation and behavioural responses of F1 larvae were different to that of F1 control larvae. Consequences on fish abilities (e.g. predator escape, food search, courtship) will be discussed.