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Fœtal programming and epigenetics: how important are they for the equine species?

Abstract : Single layer centrifugation (SLC) with Androcoll-E™ enables a better sub-population ofspermatozoa to be selected from stallion ejaculates. Installions with fertility problems, SLC-treated sperm seems to result in normal pregnancy rates. Since no studies have been performed on the effect of Androcol!-ETM treatment on pregnancy rates obtained from fertile stallions, this study was designed to investigate whether SLC could be used to enbance fertility rates of normal stallion. Ejaculates obtained from a SIRE were used to study quality and longevity of the sperm recovered after centrifugation in Androcol!-ETM as weil as pregnancy rates obtained after AI with and without SLC-centrifuged sperm. The sperm's characteristics not recovered by SLC-treatment were also investigated and results were compared by ANOVA. SLC-recovered sperm presented the highest percentage oflive cells and membranes osmotically active (HOS test) while the highest percentage of sperm with high mitochondrial potential (il'JI mit; JC-1) and DNA integrity (SCD test) was retained in the upper layer. Sperm motility in SLC-selected samples cooled for 72 h didn't differ from non­ SLC-selected sperm. A crosstabs procedure (chi-square test, x2) was used and per cycle fertility obtained with Androcol!-ETM treated semen (78%, 9 mares; 7 cycles DG+) didn't differ (x2(2)00; P=0.527; n=18) from per cycle fertility obtained with non SLC-treated sperm (89%; 9 mares; 8 cycles DG+). In conclusion, our results suggest thal fertility is independent oftreatment with Androcol!-ETM. Answer to REviwers: We have no data with sub fertille stallions regarding in vivo and in vitro comparison.
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https://hal.inrae.fr/hal-02747419
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Submitted on : Wednesday, June 3, 2020 - 11:53:26 AM
Last modification on : Thursday, August 5, 2021 - 3:55:02 AM

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  • HAL Id : hal-02747419, version 1
  • PRODINRA : 226780

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Pascale Chavatte-Palmer, Pauline Peugnet, Hélène Jammes. Fœtal programming and epigenetics: how important are they for the equine species?. 64. Annual Meeting of the European Federation of Animal Science (EAAP), European Association for Animal Production (EAAP). ITA., Aug 2013, Nantes, France. 660 p. ⟨hal-02747419⟩

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