ln this project we intend to develop the resources necessary for high throughput QTL studies in tomato by combining the development of genomic and plant resources including: (1) a large set of SNPs in expressed sequences and flanking regions based on high throughput sequencing technology. (2) a set of 4 Illumina VeraCode chips carrying a total of 1536 SNP (3) Creation and phenotype characterisation of a multiparent advanced generation intercross (MAGIC) population to be used for QTL mapping and latter for fine mapping, as a complement to genetic resources gathered in collections. The population will be characterised for yield related traits and fruit quality (composition in sugars and acids, colour, texture). (4) Differentiai Gene Expression profiles and proteome profiles of the 8 parental lines of the MAGIC populations and the 4 Fl hybrids These resources are developed to be used in the subsequent analyses: (1) Genotyping a biallelic population in order to precisely map ail the markers present on the VeraCode chips (2) Genotypinç the MAGIC population with the Illumina VeraCode chips in order to map QTL (3) Genotyping several genetic resources (collections of cherry tomato cultivars, of old cultivars and a set of elite genotypes), in order to assess linkage disequilibrium and search for associations between SNP markers and fruit related traits (4) Expression profile analysis of the genes and proteins characterised at the 2 stages of expression; comparison among genotypes and Fl hybrids. The final expected result is a genetic map of more than 1000 SNPs which will be associated with QTL locations and expression variations of the parental lines and Fl hybrids.