Because direct environmental impacts of fisheries can hardly be assessed using conventional methods of Life Cycle Assessment(LCA), we suggest building a new methodological framework to account for most of them. We propose a regionalized method ofcalculation for characterisation factors dedicated to an uptake of biomass through fishing activities (biotic resources extraction impactassessment). These characterisation factors are proposed for the assessment of impacts on biotic resources depletion and on life supportfunctions of marine ecosystems. The method is applied on two examples of fisheries, to demonstrate that it is relevant for comparisonsbetween different fisheries, exploiting different fish species. A discussion on the compatibility of this method with otherframeworks is then performed.