The effects of agricultural practices on floristic composition of grasslands are well documented but only few studies investigated these effects on plant spatial patterns involved in the maintenance of plant diversity. In order to identify major processes controlling dispersal patterns of six grassland species, presenting several dispersion modalities (anemochorous, barochorous and zoochorous sensu Grime et al., 1988), we used spatial analysis adapted to grids sampling. With these methods, when the quadrat variances are plotted against block size, the peaks of these graphs are interpreted as the scale of the mapped phenomena (Guo and Kelly, 2004). An effect of mowing on spatial patterns from functional traits connected to dispersion was shown by spatial analysis. For example, dispersal distances of barochorous species such as Ranunculus bulbosus is enhanced by mowing and grazing. In contrast, a zoochorous grass (Festuca arundinacea) which has the theoretical capacities to produce expansive lateral spread up to 1 meter was limited in its expansion by cutting and grazing. This study showed that disturbance can play an important role in dispersal and the spatial structure of grasslands species.