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Communication dans un congrès

Histological study of shoot organogenesis in melon (Cucumis melo L.) after genetic transformation

Abstract : Leave and cotyledon explants from two melon genotypes ‘Vedrantais’ and ‘Paul’ were cultured on different media and their regeneration was followed during 60 days. High frequency of plant regeneration was observed with ‘Vedrantais’ cotyledons cultured on MS medium added with BAP and 2-iP. Using this protocol, Agrobacterium mediated transformation was developed and a significant reduction of the plant regeneration rate was observed. Explants histological analysis revealed the presence of transformed meristematic areas but a rapid and premature cell vacuolization was observed. During plant regeneration, meristematic cells were affected by important cytological damages: cell dislocation accompanied by cell death and final collapsus. This disruption of meristematic areas may be related to an effect of A. tumefaciens transformation on melon cells and would explain the difficulties encountered to regenerate plants.
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Communication dans un congrès
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https://hal.inrae.fr/hal-02756821
Déposant : Migration Prodinra <>
Soumis le : mercredi 3 juin 2020 - 23:25:09
Dernière modification le : vendredi 12 juin 2020 - 10:43:26

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  • HAL Id : hal-02756821, version 1
  • PRODINRA : 21474

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Veronique Chovelon, Veronique Restier, Catherine Dogimont, Jawad Aarrouf. Histological study of shoot organogenesis in melon (Cucumis melo L.) after genetic transformation. 9. Eucarpia Meeting on Gentics and Breeding on Cucurbitaceae, May 2008, Avignon, France. ⟨hal-02756821⟩

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