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Determination and partial purification of phosphatases and phytases enzymes in rhizobia strains isolated from Phaseolus vulgaris

Abstract : The impact of phosphorus on plant growth and symbiotic N2 fixation in common bean (P. vulgaris) plants was investigated in previous work with the estimation of phosphatase and phytases activities in nodules. The interactions between common bean genotypes and rhizobia strains were studied under controlled conditions in hydroponic culture. Three strains were isolated from nodules of common bean plants collected from farmer’s fields in the Marrakech region. The fourth strain was Rhizobium tropici CIAT899 used as a reference. Bean plants inoculated with these local rhizobial strains showed higher nodulation and an increase in phytase and phosphatase activities in their nodules under phosphorus sub-deficiency especially for RhM11 strain. The studied bean-rhizobia symbioses differ in their adaptation to phosphorus sub-deficiency conditions. We have not distinguished if the enzyme activity derived from the plant cell fraction of nodules or from the rhizobia. The present work was established to determine if the rhizobia strains possess the ability to produce phosphatases and phytases. Four strains isolated from Marrakech region were studied (RhM11, RhM13 and RhM14) in comparison with Rhizobium tropici CIAT 899 used as reference strain. The phosphatase activity was established by the method of Asmar and Gissel-Nielsen (1997), whereas the phytase activity was determined according to the method of Araujo et al. 2008. The strains were able to produce acid phosphatases and phytases. Their ability to produce phosphatases was higher than production of phytases. The extracellular phosphatase activity is very low compared to that produced by intracellular phosphatase in all strains. The highest phosphatase activity was noted for RhM11 and CIAT 899 strains (5.40 nmol min-1 g-1 and 4.62 nmol min-1 g-1; respectively). It may be concluded that the studied rhizobia strains play an important role in P. vulgaris-rhizobia symbiosis under phosphorus subdeficiency conditions.
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Déposant : Migration Prodinra <>
Soumis le : mercredi 3 juin 2020 - 23:56:08
Dernière modification le : jeudi 2 juillet 2020 - 14:01:21


  • HAL Id : hal-02757155, version 1
  • PRODINRA : 268333


Btissam Mandri, Jean-Jacques Drevon, Claude Plassard, A. Peix, Adnane Bargaz, et al.. Determination and partial purification of phosphatases and phytases enzymes in rhizobia strains isolated from Phaseolus vulgaris. MICROBIOD 2009 : Biotechnologie Microbienne au service du Développement, Nov 2009, Marrakech, Morocco. 2009. ⟨hal-02757155⟩



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