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Functional genomics of pre-slaughter stress responses in chicken muscle

Abstract : Preslaughter conditions affect meat quality. However, the underlying molecular mechanisms are poorly investigated. In the present study, genomic tools were used to assess in an integrative way the biological response of chicken muscle to a stress situation before slaughter. Thigh muscles (tensor fascia latae and biceps femoris) were sampled immediately after sacrifice in standard broilers which were individually exposed (stressed) or not (control) to a restraint-transport (2h) combination stress. The analysis of transcripts using oligonucleotide microarrays show that the expression of 55 genes was affected by this stress. Proteomic analysis showed differential expression of 35 identified proteins. Integrate analysis of these data using Ingenuity System© proposes a significant network corresponding to small molecule biochemistry and cytoskeletal organisation. In addition, differential expressions of genes and proteins consistently indicate an inhibition of muscle glycolysis in response to stress. The analysis of metabolic profiles using metabolomics technique confirms the results obtained on genes and proteins since stressed animals showed lower relative quantities of the first metabolites of glycolysis.
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https://hal.inrae.fr/hal-02758191
Déposant : Migration Prodinra <>
Soumis le : jeudi 4 juin 2020 - 03:14:12
Dernière modification le : mardi 25 août 2020 - 11:30:05

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  • HAL Id : hal-02758191, version 1
  • PRODINRA : 30482

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Caroline Molette, Dominique Hazard, Wadih-Moussa Ziad, Fabien Létisse, Jérémy Pinguet, et al.. Functional genomics of pre-slaughter stress responses in chicken muscle. 19. WPSA European Symposium on the Quality of Poultry Meat, Jun 2009, Turku, Finland. ⟨hal-02758191⟩

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