Gene expression profiles in response to Nitrogen nutrition in Vitis vinifera L.
Résumé
'Cabernet Sauvignon' grapevines were grown under full light and high (7.1 mM) and low (1.2 mM) nitrogen supply from fruit set to maturity. Six Suppressive Subtractive Hybridization (SSH) libraries have been produced from mRNA extracted from the roots, leaves and berries grown in the two N treatments. In a first step towards the characterization of N-responsive genes in grapevine, about 450 clones randomly selected among the 16 000 clones picked up from the 6 SSH libraries have been sequenced. Functional categorization of these candidate N-responsive genes from leaves, roots and fruit shows a differential tissue response to N nutrition. Many candidate N response genes are unknown or have an unidentified function (36%). In conclusion about 334 candidate N-response genes were selected. They will be used for screening the expression pattern related to N availability of grapevines in the vineyards.