Four pairs of simple sequence repeat (SSR) primers were used to perform an analysis of the level of genetic diversity among 30 natural Scots pille provenances. Two microsatellite loci (SP AC 7.14 and SP AC 12.5) - which were previously developed for Pinus sylvestris, and two microsatellite loci Rptest11 and SsrPt-ctg4363 from P. pinaster were investigated for genotype Scots pine populations in Poland. PCR amplifications were analyzed with an automatic analyzer sequencer, and the occurrence of non-expected lengths of alleles for the two loci SPAC 7.14 and SsrPt-ctg4363 was observed. S-Plus software version 3.4 was then used to determine the accurate allele fragment length at each locus. A total of 112 alleles were detected, with average heterozygosity coefficients of He = 0.803 and Ho = 0.820 per locus. A low level for mean genetic diversity values (Fis=0.0168, Fit= 0.0187 and Fst = 0.002) was characteristic of Polish Scots pine provenances, and no spatial correlation between the observed gene diversity and geographical location of provenances bas been found. The dendrogram for Nei's genetic distances showed that the studied provenances could be grouped into two main clusters - with the exception of the single provenance constituting the Primeval Forest of Bialowieza National Park. The impact of human activity in the transfer of Scots pine seed in the past bas been discussed as it concerns these data.