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Communication dans un congrès

Molecular cloning of a cDNA encoding a putative cuticle collagen of Trichinella spiralis

Abstract : 5-day-old adult stage-specific cDNA fragment from Trichinella spiralis was identified by suppression subtractive hybridization and was used as a probe to screen the cDNA library. The cDNA sequence coding for a putative T spiralis cuticle collagen was isolated. The cDNA encoded an open reading frame of 343 amino acid residues with molecular weight of 35.1 kDa. The deduced protein contained an N-terminal signal peptide, a nematode cuticle collagen N-terminal domain and a collagen triple helix repeat domain. Searches in GenBank using BLASTP showed up to 47% identity to cuticle collagens from other nematodes. Southem blot analysis of genomic DNA indicated this gene was present as a single copy in T spiralis genome.
Mots-clés : CDNA CUTICLE COLLAGEN
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Communication dans un congrès
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https://hal.inrae.fr/hal-02764309
Déposant : Migration Prodinra <>
Soumis le : jeudi 4 juin 2020 - 07:54:02
Dernière modification le : mardi 18 août 2020 - 17:00:19

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B.Q. Fu, M.Y. Liu, C.M.O. Kapel, X.P. Meng, Q. Lu, et al.. Molecular cloning of a cDNA encoding a putative cuticle collagen of Trichinella spiralis. 11. International Conference on Trichinellosis, Aug 2004, San Diego, United States. ⟨10.1016/j.vetpar.2005.05.020⟩. ⟨hal-02764309⟩

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