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Communication dans un congrès

Simple species identification of Trichinella isolates by amplification and sequencing of the 5S ribosomal DNA intergenic spacer region

Abstract : We developed a PCR-based assay using a single primer pair to amplify the 5S ribosomal DNA intergenic spacer region to identify Trichinella isolates. In our method, amplified products are directly sequenced on both strands and compared to GenBank sequences. Using this method, we were able to identify Trichinella spiralis, T britovi and T nativa. This method permits rapid species identification of Trichinella isolates; however, further evaluation is required before recommending this approach for routine use.
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Communication dans un congrès
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https://hal.inrae.fr/hal-02764506
Déposant : Migration Prodinra <>
Soumis le : jeudi 4 juin 2020 - 08:03:42
Dernière modification le : mercredi 19 août 2020 - 11:19:08

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Aymeric de Bruyne, Hélène Yera, Franck Le Guerhier, Pascal Boireau, Jean Dupouy-Camet. Simple species identification of Trichinella isolates by amplification and sequencing of the 5S ribosomal DNA intergenic spacer region. 11. International Conference on Trichinellosis, Aug 2004, San Diego, United States. ⟨10.1016/j.vetpar.2005.05.026⟩. ⟨hal-02764506⟩

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