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Communication Dans Un Congrès Année : 2005

Simple species identification of Trichinella isolates by amplification and sequencing of the 5S ribosomal DNA intergenic spacer region

Résumé

We developed a PCR-based assay using a single primer pair to amplify the 5S ribosomal DNA intergenic spacer region to identify Trichinella isolates. In our method, amplified products are directly sequenced on both strands and compared to GenBank sequences. Using this method, we were able to identify Trichinella spiralis, T britovi and T nativa. This method permits rapid species identification of Trichinella isolates; however, further evaluation is required before recommending this approach for routine use.

Dates et versions

hal-02764506 , version 1 (04-06-2020)

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Citer

Aymeric de Bruyne, Hélène Yera, Franck Le Guerhier, Pascal Boireau, Jean Dupouy-Camet. Simple species identification of Trichinella isolates by amplification and sequencing of the 5S ribosomal DNA intergenic spacer region. 11. International Conference on Trichinellosis, Aug 2004, San Diego, United States. ⟨10.1016/j.vetpar.2005.05.026⟩. ⟨hal-02764506⟩
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