In this study the genetic diversity of five selected French dairy sheep subpopulations and breeds was quantified. Three different inbreeding estimation methods were assessed: a pedigree-based methodology, a single SNP-based approach and a ROHbased method. The data set available included individuals from the Basco-Béarnaise (BB) breed, Manech Tête Noire (MTN) breed, Manech Tête Rousse (MTR) breed, Lacaune Confederation (LACCon) subpopulation and Lacaune Ovitest (LACOvi) subpopulation. Animals were genotyped with the Illumina OvineSNP50 BeadChip. After filtering, the genomic data included 38,287 autosomal SNPs and 8,700 individuals. Pedigree from the genotyped animals comprised 72,803 animals. The results indicate that no significant differences were observed in e N estimates between the three inbreeding estimation methods. In general, estimates of e N were above 200 in LACCon and LACOvi subpopulations and below 200 in BB, MTN and MTR breeds.