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 Design, development and validation of a real-time PCR assay for detection of <em>Klebsiella pneumoniae</em> complex in environmental matrixes Elodie Barbier e432932af17e7006d4b7a4b02a1d7527 inra.fr 1136559 378873-1136559 C. Rodrigues 1184121-0 Géraldine Depret e2d26dc9b5c0aa1e104a4810d04b9664 inra.fr 1207385 891849-1207385 V. Passet 1523482-0 Laurent Gal ee68ea3c8170590c0aae89502fae4c97 agrosupdijon.fr laurent-gal 171716 26620-171716 http://www.researcherid.com/rid/A-2824-2011 https://www.idref.fr/087398974 https://orcid.org/0000-0001-6598-7262 http://www.researcherid.com/rid/http://www.researcherid.com/rid/A-2824-2011 Pascal Piveteau 5ba9b5b57019acf8d54e06fdbaa3ed5b inrae.fr pascal-piveteau 747916 48635-747916 https://orcid.org/0000-0002-1210-251X https://www.idref.fr/110492064 S. Brisse 7461e3fba3ca97bdf96c679963053c31 pasteur.fr sylvain-brisse 744912 497311-744912 https://orcid.org/0000-0002-2516-2108 https://www.idref.fr/133552845 Migration ProdInra 9e5ed7d56b52b3d4383123b1ffb42236 inrae.fr 2021-10-18 08:50:12 2025-03-31 08:12:06 2021-11-17 13:09:03 2019-05-22 2021-10-18 inra_publicVise_local_SC contributor Migration ProdInra 9e5ed7d56b52b3d4383123b1ffb42236 inrae.fr CCSD hal-02787597 https://hal.inrae.fr/hal-02787597 barbier:hal-02787597 <i>1. One Health European Joint Programme Annual Scientific Meeting</i>, May 2019, Dublin, Ireland. <a target="_blank" href="https://www.ohejp2019.com/"></a>, 2019 1. One Health European Joint Programme Annual Scientific Meeting, May 2019, Dublin, Ireland. , 2019 HAL Authorization Institut Pasteur Sciences De l'Environnement Université Bourgogne Europe INRA - Institut national de la recherche agronomique GIP Bretagne Environnement Archive ouverte en agrobiosciences AGROSUP-DIJON Institut National de Recherche en Agriculture, Alimentation et Environnement Institut Agro Dijon - AE Institut Agro Dijon Institut Agro - AE Agroécologie Institut Agro Publications des enseignants chercheurs de l'Institut Agro Prod 2019-104a Equipe EA BIOmE UB AGROSUP International No No Yes No Design, development and validation of a real-time PCR assay for detection of <em>Klebsiella pneumoniae</em> complex in environmental matrixes Elodie Barbier e432932af17e7006d4b7a4b02a1d7527 inra.fr 1136559 378873-1136559 C. Rodrigues 1184121-0 Géraldine Depret e2d26dc9b5c0aa1e104a4810d04b9664 inra.fr 1207385 891849-1207385 V. Passet 1523482-0 Laurent Gal ee68ea3c8170590c0aae89502fae4c97 agrosupdijon.fr laurent-gal 171716 26620-171716 http://www.researcherid.com/rid/A-2824-2011 https://www.idref.fr/087398974 https://orcid.org/0000-0001-6598-7262 http://www.researcherid.com/rid/http://www.researcherid.com/rid/A-2824-2011 Pascal Piveteau 5ba9b5b57019acf8d54e06fdbaa3ed5b inrae.fr pascal-piveteau 747916 48635-747916 https://orcid.org/0000-0002-1210-251X https://www.idref.fr/110492064 S. Brisse 7461e3fba3ca97bdf96c679963053c31 pasteur.fr sylvain-brisse 744912 497311-744912 https://orcid.org/0000-0002-2516-2108 https://www.idref.fr/133552845 1. One Health European Joint Programme Annual Scientific Meeting 2019-05-22 2019-05-24 Dublin Ireland 2019 487088 https://www.ohejp2019.com/ English Life Sciences [q-bio] Environmental Sciences Life Sciences [q-bio]/Vegetal Biology Poster communications Poster communications Poster communications

<em>Klebsiella Klebsiellapneumoniae</em> (Kp )represents agrowinggrowing public publichealth concern due dueto the emergence emergence of multidrug multidrug multidrug resistance resistance and hypervirulence hypervirulence . Seven closely related relatedphylogroups constitute constitute the Kp species species speciescomplex complex :<em>Klebsiella Klebsiellapneumoniae pneumoniae pneumoniae</em> (phylogroup (phylogroup Kp 1), <em>K.quasipneumoniae quasipneumoniae subsp</em> .<em>quasipneumoniae (Kp 2)</em>and <em>subsp .similipneumoniae similipneumoniae similipneumoniae similipneumoniae similipneumoniae (Kp 4)</em>, <em>K.variicola variicola variicolasubsp .variicola variicola(Kp 3)and subsp</em> <em>.tropicalensis tropicalensis (Kp 5), K.quasivariicola <em>quasivariicola ’ (Kp 6)and K.africanensis africanensis (Kp 7)</em>.</em> Kp is describeddescribed described described as ubiquitousubiquitous ubiquitous ubiquitousin naturenature .Water,Water, Water,sewage, soil and vegetation could be environmental environmental sources of Kp but their exact contribution contributioncontribution contributionin its itstransmission transmission routesroutes is still unknown . Objective Objective :To develop developdevelop aspecific specific specific and sensitive sensitive sensitive sensitivereal time PCR assay for the detection detection of all phylogroups phylogroups (Kp 1to Kp 7)of the Kp complex from environmental sources sources.

 159749875 201220381F Agroécologie [Dijon] 2017-01-01 2019-12-31
Agroécologie, AgroSup Dijon, INRAE, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France
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