Since its introduction in 2004 in France the asian yellow-legged hornet Vespa velutina nigrithorax is now largely spreading into Europe, by lack of efficient control methods. In this context, looking for natural hornet pathogens could be useful to develop biological control agents against this invasive species. Therefore, we investigated all RNA viral sequances detected from hornets samples caught in Southern France, in order to characterize any virus from asymptomatic or symptomatic hornets differentiating various dissected parts (brain, muscle, abdomen). Among almost twenty viruses detected in three RNA sequenced hornets, Deformed wing virus B was shown to be predominant in all samples, and much higher in the muscle from symptomatic individual, suggesting a putative cause of the deformed wings symptoms. Complete genomes of Acute bee paralysis virus (ABPV, Dicistroviridae, Aparavirus) and Black queen cell virus ( BQCV, Dicistroviridae, Triatovirus) were found in the intestine, and multiplex PCR detection confirmed these viruses were present in different dissected parts of several hornets. Five other viruses known to infect honeybees were also partially sequenced. Eleven other insect viruses were detected in hornets, most of them belonging to picornavirales order, but some of them resulted as new species and possibly new genus. Nine of them were detected only in the intestine transcriptome, and not in brain or muscle tissues, suggesting they could originate from infected preys ingested by hornet, and not by infected hornets thenselves and thus giving indirect data about hornet diet. The transmission routes from or to V. velutina have to be investigated to better assess the impact they may have on virus dissemination in European honeybee populations. Our study underlines the urgent need to study the host range of these original newly discovered virus that we described in hornets to evaluate if they can represent a new threat for the honeybees or a hope for the biocontrol of V. velutina.