, However, these species are threatened by extinction arising from pressure on their genetic diversity, over-utilisation and difficulty of their propagation through conventional methods. Therefore, effect of priming using water and different growth media on germination of P. angolensis and Z. zanthoxyloides seeds were investigated.Two hundred and forty mature fruits of P. angolensis and Z. zanthoxyloides were collected from Nigeria. One hundred and twenty seeds of each species were soaked in deionised water for 24 hours while the rest were not. Seeds were sterilized with 70% ethanol for two minutes and 50% Clorox for five minutes before inoculation. Murashige and Skoog (MS), woody plant medium (WPM) supplemented with 10 mg/L Indole Butyric Acid (IBA), 0.5-5.0 mg/L 6-Benzyl Amino Purine (BAP), and 5.0 mg/L kinetin (KIN) were combined for in vitro seed germination. Cultured bottles were arranged in Completely Randomised Design (CRD) and replicated four times. The setup was observed fortnightly to determine the onset of germination. Data were analysed using descriptive statistics and ANOVA at ?=0.05. Priming of seeds in deionized water enhanced germination of P. angolensis seeds. Germination at 14.00±16.24 days were observed in soaked seeds, Pycnanthus angolensis (Welw.) and Zanthoxylum zanthoxyloides (Lam.) are tree species with great importance in traditional ethnomedicine

. Mg/l-kin+10-mg/l-iba, In vitro seed germination of Pycnanthus angolensis requires priming with water which enhanced germination

, DPlant genetics & forestry managementCIRAD-Biological Systems DepartmentResearch unit Genetic improvement and adaptation of mediterranean and tropical plantsCampus of Lavalette, Montpellier -FRANC-Dr Jean-Marc BouvetPlant genetics & forestry managementCIRAD-Biological Systems DepartmentResearch unit Genetic improvement and adaptation of mediterranean and tropical plantsCampus of Lavalette

, This species has been introduced in Madagascar in the end of the 19th century. The plantations established by smallholders are characterized by a low genetic diversity and a high level of inbreeding. There is a need to create a new breeding population to start a new improvement program and produce the future varieties able to meet the woods demand, A provenance trial including 1314 individuals from 19 provenances covering the natural range of the species in Australia has been Comparative analysis of gene dispersal based on SSR and SNP markers

. Chybicki-ij-1* and A. Piotti, Vendramin GG, vol.2, issue.2

, Biosciences and BioResources, vol.30

, Thünen Institute of Forest Genetics

G. Alterra, W. Research, R. University, and . Centre, And while polyphenols can preserve DNA from degradation via the inhibition on nucleases, equally, in the presence of metal ions, they can contribute to DNA degradation. For these reasons, effective protocols to obtain polyphenol-free DNA extracts are desirable. We are testing ascorbic acid, ?-mercaptoethanol and polyvinylpyrrolidone (PVP) for their ability to reduce content and oxidation of polyphenols during oak heartwood DNA extraction

F. Aravanopoulos and M. Westergren, Bozic G(b), Kavaliauskas D(c), Finzgar D(b), Baier R(c), Barbas E (a), Malliarou H(a), Ganopoulos I(a),Bekiaroglou P (d), Hasilidis P(d), Andonovski V(e), Ballian D(f), Bordács S(g) Kajba D(h), Konrad H(i), Orlovi? S(j), Kiourtsis F(d), Veseli? Z(k), Konnert M(c)

, Slovenian Forestry Institute, Slovenia, (b)Bavarian Office for Forest Seeding and Planting

S. Forest-service,

A. Pérez-gonzález1, M. Marconi2, and P. Perdiguero3,

G. Departamento-de, Facultad de Ciencias Biológicas, vol.12, p.28223, 28040.

G. I. Genfor, Genética y Fisiología Forestal, p.28040

D. De and S. Físicos, Ctra. Utrera, km, vol.1, p.41013

. Linares, ), in order to identify characterized genes involved in drought stress response. Several genes involved in this response has been identified, such as AP2/ERF, DREB, MYB, Heat-Shock or Dehydrogenase genes, all of them involved in drought-stress tolerance. These transcripts constitute potential candidate genes as a starting point in further studies related with abiotic stresses responses in A. pinsapo. On the other hand, the availability of this high-throughput sequencing data has led us to the discovery of specific SNPs (100,949) and SSRs (5,663), They are distributed between 1,000 and 1,800 metres in altitude, always in shady and north-facing slopes where they can receive abundant orographic rainfall, vol.1, 1971.

M. Lstiburek, Shawn Mansfield -IUFRO subdivision 2.4.6 coordinator "Molecular biology of forest trees, Nathaniel Street -IUFRO

J. Jaramillo-correa, S. González-martínez, C. Lexer, and X. Wang,

V. Segura, S. Aitken, A. Eckert, and Y. Tsumura,

J. Cooke, G. L. Provost, F. Martin, and I. Yakovlev,

A. Hipp, M. Heuertz, and J. Bousquet, Jérôme Salse Session, vol.5

B. Fady, K. Kramer, D. Grattapaglia, and F. Isik, Laurent Bouffier Session, vol.6

N. Street, G. Dutkowski, J. Mackay, and J. Aury, Ross Whetten GenomicSelection Workshop Léopoldo Sanchez

B. Brachi, S. Wagner, T. Leroy, G. L. Provost, L. Bouffier et al.,

A. Franc, . Biogeco, U. Inra, and . Bordeaux,

N. Francillonne and . Nicolas,

B. Fussi, Bavarian Office for Forest Seeding and Planting (ASP) -Germany barbara

. Gailing,

G. Rodriguez, F. Javier, and U. Fjgalleg@ucm, es Gamal Ibrahim, Omnia University of British Columbia -Canada omnia

S. Gardet, . Biogeco, U. Inra, and . Bordeaux,

M. Gebreselassie and . Inra--france,

P. Garnier-géré, . Biogeco, U. Inra, and . Bordeaux,

G. Pérez, Gustavo Ibrahim Instituto de Ecología, UNAM -México tavoibrahim@gmail

P. Goicoechea and . Neiker--spain-pgoikoetxea@neiker,

G. Lopes, M. Teresa-ufam--brazil-mtglopes@pq.cnpq.br-gonzález, E. Futuragene--brazil-esteban@futuragene, S. Gonzalez-martinez, . Biogeco et al., Gonzalez-Martinez@pierroton.inra.fr Grima-Pettenati

O. Rogier and . De-loire,

. Sofletea,

T. Solvin, N. Mørtvedt, and . Thomas,

J. Soularue, . Biogeco, U. Inra, and . Bordeaux,

. St and . Onge,

Z. R. Stangl,

M. Staton,