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Genome-wide analysis of methylation in bovine clones by methylated DNA immunoprecipitation (MeDIP)

Abstract : Methylated DNA immunoprecipitation (MeDIP), when coupled to high-throughput sequencing or microarray hybridization, allows for the identification of methylated loci at a genome-wide scale. Genomic regions affected by incomplete reprogramming after nuclear transfer can potentially be delineated by comparing the MeDIP profiles of bovine clones and non-clones. This chapter presents a MeDIP protocol largely inspired from Mohn and colleagues (Mohn et al., Methods Mol Biol 507:55–64, 2009), with PCR primers specific for cattle, and when possible, overviews of experimental designs adapted to the comparison between clones and non-clones.
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Submitted on : Friday, June 5, 2020 - 2:57:07 PM
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Hélène Kiefer. Genome-wide analysis of methylation in bovine clones by methylated DNA immunoprecipitation (MeDIP). Nuclear reprogramming: Methods and Protocols, 1222 (2. ed), Editions Springer, 290 p., 2015, Methods in Molecular Biology, 978-1-4939-1593-4. ⟨10.1007/978-1-4939-1594-1_20⟩. ⟨hal-02797611⟩

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