A novel platform based on immobilized olfactory receptors, for detection odorant molecules characteristics of boar taint
Résumé
We report a dose-dependent detection of androstenone in solution, as one of boar taint compounds, based on related OR 7D4 olfactory receptors immobilized on gold electrode through their 6-His tag and NTA-copper complex (Scheme 1), as visualized through fluorescence microscopy. Square wave voltammetry (SWV) is the method used to monitor the electrochemical transduction process (Fig. 1). The relative variation of current Cu(I)-Cu(II) peak increases linearly versus log (concentration of androstenone) from 10-14M to 10-4M, in buffer solution. Negative tests were performed, using an unrelated odorant, helional, itself related a ligand of OR 1740. Cross-selectivity was also tested after immobilization of OR 1740.