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A novel platform based on immobilized olfactory receptors, for detection odorant molecules characteristics of boar taint

Abstract : We report a dose-dependent detection of androstenone in solution, as one of boar taint compounds, based on related OR 7D4 olfactory receptors immobilized on gold electrode through their 6-His tag and NTA-copper complex (Scheme 1), as visualized through fluorescence microscopy. Square wave voltammetry (SWV) is the method used to monitor the electrochemical transduction process (Fig. 1). The relative variation of current Cu(I)-Cu(II) peak increases linearly versus log (concentration of androstenone) from 10-14M to 10-4M, in buffer solution. Negative tests were performed, using an unrelated odorant, helional, itself related a ligand of OR 1740. Cross-selectivity was also tested after immobilization of OR 1740.
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https://hal.inrae.fr/hal-02799314
Contributor : Migration Prodinra <>
Submitted on : Friday, June 5, 2020 - 5:31:12 PM
Last modification on : Thursday, July 22, 2021 - 3:48:58 AM

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  • HAL Id : hal-02799314, version 1
  • PRODINRA : 267738

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Zhenzhong Guo, Nadia Zine, Florence Lagarde, Karine Badonnel, Marie-Annick Persuy, et al.. A novel platform based on immobilized olfactory receptors, for detection odorant molecules characteristics of boar taint. 3. International Conference and Exhibition on Biosensors & Bioelectronics, OMICS Group Conferences., Aug 2014, San Antonio, United States. ⟨hal-02799314⟩

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