Fruit of a continuous dialogue between ARIMNet2 partners (i.e. 24 funding agencies and research institutions), renowned international scientists and European institutions, and based on a shared vision of Mediterranean challenges and priorities, the ARIMNet2 Integrated Strategic Research Agenda (ISRA) constitutes a transnational strategy to boost agricultural research and innovation and enhance sustainably food security, natural resource preservation and socio-economic development across the Mediterranean region. The ISRA is the actual roadmap for joint action in the framework of ARIMNet2 and might also guide future similar initiatives and policy-making. It frames the multiple activities implemented or proposed by the ARIMNet2 Consortium to actively support Mediterranean research and innovation efforts, notably by funding collaborative research projects (e.g. transnational calls), sharing research infrastructures and equipment (e.g. joint experiments, shared platforms), and fostering knowledge exchange and capacity-building (e.g. young researchers seminars, international conferences, networking events). The ISRA builds upon and complements existing European initiatives for the Mediterranean region, in particular the FACCE-JPI and Standing Committee on Agricultural Research (SCAR), paving the way for a more inclusive and effective Euro-Mediterranean cooperation on agricultural research and innovation.