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Communication Dans Un Congrès Année : 2011

Why do highly similar Salmonella serotypes present very different pathogenies? Study of cell invasion impact

Résumé

Salmonella enterica includes serotypes with a large range of hosts, as Salmonella Enteritidis, and high restricted host serotypes, as Salmonella Gallinarum, pathogen for avian species. The aim of this study is to understand why these two serotypes show very different patterns although they share 99.7 % of nucleotide identity between orthologs genes. Preliminary epithelial cell infection assays showed that Salmonella Gallinarum is poorly invasive compared to Salmonella Enteritidis. In vivo experiments demonstrated that S. Enteritidis strain LA5 (SELA5) is responsible for asymptomatic carriage in poultry whereas S. Gallinarum strain 287/91 (SG287/91) is lethal. S. Gallinarum strain 2210 (SG2210) and S. Gallinarum strain 9S (SG9S) were also tested. No lethality was observed. Complete genome of SELA5, SG221 0 and SG9S were sequenced, genome of 287/91 is available on line. Global genome comparison showed very high similarity. Some large insertions/deletions exist between SELA5 and S. Gallinarum strains, analysis of these regions is in process. Sequence analysis revealed especially point mutations in four main effectors of the Type Three Secretion System-1 (T3SS-l), a secretion system involved in non-phagocytic cell invasion. We have demonstrated, however, that S. Gallinarum was able to secrete in vitro the T3SS-l effectors. To demonstrate that these mutations explain the low invasion rate, we will complement the SG287/91 strain with the SELA5 T3SS-l effectors genes. Because the organ responsible for host invasion by S. Gallinarum is not yet clearly identify, we will also build a tagged SG287/91 strain to follow in vivo this strain in order to identify the organ infected and then the cells in vivo infected.

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Dates et versions

hal-02809343 , version 1 (06-06-2020)

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  • HAL Id : hal-02809343 , version 1
  • PRODINRA : 50873

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Aurore Rossignol, Pierrette Menanteau, Jérôme Trotereau, Nadia Abed, Olivier Grépinet, et al.. Why do highly similar Salmonella serotypes present very different pathogenies? Study of cell invasion impact. 2011 PhD Day, Oct 2011, Nouzilly, France. 1p. ⟨hal-02809343⟩
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